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ProSwiftRP蛋白質色譜柱ProSwiftRP蛋白質色譜柱 Thermo Fisher

ProSwiftRP蛋白質色譜柱ProSwiftRP蛋白質色譜柱

  • 產品綜述

 ProSwift RP 蛋白質色譜柱

  整體反相色譜柱，用于高流速狀態(tài)下高分辨蛋白質分離。
  Monolith Reversed-Phase Columns for High-Resolution Protein Separations at High Flow Rates

概述:
ProSwift® reversed-phase monolith columns uniquely provide the advantages of high resolution at exceptionally high flow rates for protein separations. They deliver the outstanding resolving power of nonporous analytical media combined with faster separations than any bead based columns available. ProSwift polymer monolith columns achieve long column lifetime, exhibit reproducibility even after hundreds of runs, and have broad pH stability, providing high stringent wash capability.

  • High resolution at high speed
  • Highest operational flow rates available
  • High throughput and improved productivity
  • Optimal performance in a broad range of applications
  • Excellent stability over a wide pH range of 1 to 14
  • Outstanding reproducibility and ruggedness
  • High stringent wash compatible, for example, 1 M NaOH
  • High loading capacity

  ProSwift polymer monolith media are uniquely suited for the separation of proteins. Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels and the monolith’s nonporous surfaces result in fast mass transfer for high-resolution and fast protein separations. The channels also produce low backpressure, allowing the use of higher linear velocities with minimal loss of resolution.

規(guī)格:
ProSwift Reversed-Phase Monolith Columns Specifications
Column Name Proswift RP-15 Proswift RP-2H ProSwift RP-3U Proswift RP-4H   ProSwift RP-10R
Column Dimensions 4.6 × 50 mm 4.6 × 50 mm 4.6 × 50 mm 1 × 50 mm 1 × 250 mm 1 × 50 mm
Base Matrix Material Polystyrene-divinlbenzene
Surface Chemistry Phenyl
Protein Binding Capacity per mL of Polymer 3.0 mg/mL Lysozyme 1.0 mg/ml Lysozyme .05 mg/ml Lysozyme 2.3 mg/ml Lysozyme   n.d.*
Protein binding capacity per column 2.2 mg Lysozyme 0.8 mg Lysozyme 0.3 mg Lysozyme 0.1 mg Lysozyme 0.5 mg Lysozyme n.d.
Bed Height 46 mm 44 mm 40 mm 50 mm 250 mm 50 mm
Bed Volume （mL） 0.76 mL 0.73 mL 0.65 mL 0.039 mL 0.196 mL 0.039 mL
pH Range 1–14 1–10
Recommended Flow Rate 0.5–4 mL/min 1–10 mL/min 1–16 mL/min 0.1–0.4 mL/min 0.1–0.3 mL/min 40–60 µL/min
Maximum Flow Rate 5 mL/min 14 mL/min 20 mL/min 0.5 mL/min 0.4 mL/min 100 µL/min
Maximum Pressure 2800 psi <2000 psi 3000 psi 4350 psi
19.3 MPa 13.8 Mpa 20.7 MPa 30 MPa
Operating Pressure < 1500 psi < 2500 psi 2900 psi
10.4 MPa 17.5 MPa 20 MPa
Temperature 70 °C 80 °C
Solvent Compatability Most Common organic solvents

訂購信息：
Analytical Columns
ProSwift RP-2H Monolith Column （4.6 x 50 mm） 064296
ProSwift RP-1S Monolith Column （4.6 x 50 mm） 064297
ProSwift RP-3U Monolith Column （4.6 x 50 mm） 064298
ProSwift RP-4H Monolith Column （1.0 x 250mm） 066640
ProSwift RP-4H Monolith Column （1.0 x 50mm） 069477
ProSwift RP-10R Monolithic Capillary Column, 1.0 mm i.d. x 5 cm 164397

應用譜圖：

 

 

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