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ProPacHIC蛋白質(zhì)和羧氨酸柱ProPacHIC蛋白質(zhì)和羧氨酸柱 Thermo Fisher

ProPacHIC蛋白質(zhì)和羧氨酸柱ProPacHIC蛋白質(zhì)和羧氨酸柱

  • 產(chǎn)品綜述

 ProPac HIC 疏水性色譜柱

  ProPac HIC 疏水性色譜柱用于生物大分子的分離。
  ProPac HIC Hydrophobic Interaction Chromatography Columns for the Separation of Large Biomolecules

概要:

Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

  • Based on 5 µm ultrahigh purity spherical silica gel particles, with 300 Å pores
  • Proteins separated under non-denaturing conditions
  • High protein loading capacity for protein purification applications
  • Proprietary multidentate silica bonding for enhanced hydrolytic stability
  • High-resolution HPLC separation of proteins and peptides
  • Wide range of applications

  The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.

規(guī)格:

ProPac HIC Columns  Specifications
Protein Loading Capacity 340 mg Lysozyme per 7.8 x 75 mm column
Starting Material Ultrapure Spherical Silica
Particle Size 5 µm
Pressure Limit 4,000 psi
Column Construction Stainless Steel
Phase HIC
Average Pore Diameter 300 Å
Endcapped No
Mobile Phase Compatibility 2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup
pH range pH 2.5 – 7.5
Temperature Limit < 40 °C

訂貨信息:
Analytical Columns
ProPac HIC-10 Column (2.1 x 100 mm) 063653
ProPac HIC-10 Column (4.6 x 100 mm) 063655
ProPac HIC-10 (4.6 x 250mm) 074197
ProPac HIC-10 Column (7.8 x 75 mm) 063665


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